Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a useful tool for the rapid identification of wild flea vectors preserved in alcohol.
Identifieur interne : 000504 ( Main/Exploration ); précédent : 000503; suivant : 000505Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry as a useful tool for the rapid identification of wild flea vectors preserved in alcohol.
Auteurs : A. Zurita [Espagne] ; R. Djeghar [Algérie] ; R. Callej N [Espagne] ; C. Cutillas [Espagne] ; P. Parola [France] ; M. Laroche [France]Source :
- Medical and veterinary entomology [ 1365-2915 ] ; 2019.
Descripteurs français
- KwdFr :
- Algérie (MeSH), Animaux (MeSH), Chiens (MeSH), Espagne (MeSH), Hérissons (MeSH), Infestations par les puces (classification), Infestations par les puces (médecine vétérinaire), Maladies des chiens (classification), Manipulation d'échantillons (médecine vétérinaire), Siphonaptera (classification), Spectrométrie de masse MALDI (médecine vétérinaire), Spectrométrie de masse MALDI (méthodes), Vecteurs insectes (classification), Éthanol (MeSH).
- MESH :
- médecine vétérinaire : Infestations par les puces, Manipulation d'échantillons, Spectrométrie de masse MALDI.
- méthodes : Spectrométrie de masse MALDI.
- Algérie, Animaux, Chiens, Espagne, Hérissons, Infestations par les puces, Maladies des chiens, Siphonaptera, Vecteurs insectes, Éthanol.
- Wicri :
English descriptors
- KwdEn :
- Algeria (MeSH), Animals (MeSH), Dog Diseases (classification), Dogs (MeSH), Ethanol (MeSH), Flea Infestations (classification), Flea Infestations (veterinary), Hedgehogs (MeSH), Insect Vectors (classification), Siphonaptera (classification), Spain (MeSH), Specimen Handling (veterinary), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (veterinary).
- MESH :
- chemical : Ethanol.
- geographic : Algeria, Spain.
- classification : Dog Diseases, Flea Infestations, Insect Vectors, Siphonaptera.
- methods : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- veterinary : Flea Infestations, Specimen Handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Animals, Dogs, Hedgehogs.
Abstract
Flea identification is a significant issue because some species are considered as important vectors of several human pathogens that have emerged or re-emerged recently, such as Bartonella henselae (Rhizobiales: Bartonellaceae) and Rickettsia felis (Rickettsiales: Rickettsiaceae). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been evaluated in recent years for the identification of multicellular organisms, including arthropods. A preliminary study corroborated the usefulness of this technique for the rapid identification of fleas, creating a preliminary database containing the spectra of five species of flea. However, longterm flea preservation in ethanol did not appear to be an adequate method of storage in the context of specimen identification by MALDI-TOF MS profiling. The goal of the present work was to assess the performance of MALDI-TOF MS in the identification of seven flea species [Ctenocephalides felis (Siphonaptera: Pulicidae), Ctenocephalides canis, Pulex irritans (Siphonaptera: Pulicidae), Archaeopsylla erinacei (Siphonaptera: Pulicidae), Leptopsylla taschenbergi (Siphonaptera: Ceratophyllidae), Stenoponia tripectinata (Siphonaptera: Stenoponiidae) and Nosopsyllus fasciatus (Siphonaptera: Ceratophyllidae)] collected in the field and stored in ethanol for different periods of time. The results confirmed that MALDI-TOF MS can be used for the identification of wild fleas stored in ethanol. Furthermore, this technique was able to discriminate not only different flea genera, but also the two congeneric species C. felis and C. canis.
DOI: 10.1111/mve.12351
PubMed: 30516832
Affiliations:
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Zurita</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville</wicri:regionArea><wicri:noRegion>Seville</wicri:noRegion></affiliation></author><author><name sortKey="Djeghar, R" sort="Djeghar, R" uniqKey="Djeghar R" first="R" last="Djeghar">R. 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Laroche</name><affiliation wicri:level="3"><nlm:affiliation>Institut de Recherche pour le Développement (IRD), Assistance Publique-Hôpitaux de Marseille (AP-HM), Service de Santé des Armées (SSA), Vecteurs-Infections Tropicales et Méditerranéennes (VITROME), Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Aix Marseille University, Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Recherche pour le Développement (IRD), Assistance Publique-Hôpitaux de Marseille (AP-HM), Service de Santé des Armées (SSA), Vecteurs-Infections Tropicales et Méditerranéennes (VITROME), Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Aix Marseille University, Marseille</wicri:regionArea><placeName><region type="region">Provence-Alpes-Côte d'Azur</region><region type="old region">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author></analytic><series><title level="j">Medical and veterinary entomology</title><idno type="eISSN">1365-2915</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Algeria (MeSH)</term><term>Animals (MeSH)</term><term>Dog Diseases (classification)</term><term>Dogs (MeSH)</term><term>Ethanol (MeSH)</term><term>Flea Infestations (classification)</term><term>Flea Infestations (veterinary)</term><term>Hedgehogs (MeSH)</term><term>Insect Vectors (classification)</term><term>Siphonaptera (classification)</term><term>Spain (MeSH)</term><term>Specimen Handling (veterinary)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (veterinary)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Algérie (MeSH)</term><term>Animaux (MeSH)</term><term>Chiens (MeSH)</term><term>Espagne (MeSH)</term><term>Hérissons (MeSH)</term><term>Infestations par les puces (classification)</term><term>Infestations par les puces (médecine vétérinaire)</term><term>Maladies des chiens (classification)</term><term>Manipulation d'échantillons (médecine vétérinaire)</term><term>Siphonaptera (classification)</term><term>Spectrométrie de masse MALDI (médecine vétérinaire)</term><term>Spectrométrie de masse MALDI (méthodes)</term><term>Vecteurs insectes (classification)</term><term>Éthanol (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Ethanol</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Algeria</term><term>Spain</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Dog Diseases</term><term>Flea Infestations</term><term>Insect Vectors</term><term>Siphonaptera</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infestations par les puces</term><term>Manipulation d'échantillons</term><term>Spectrométrie de masse MALDI</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Spectrométrie de masse MALDI</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Flea Infestations</term><term>Specimen Handling</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Dogs</term><term>Hedgehogs</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Algérie</term><term>Animaux</term><term>Chiens</term><term>Espagne</term><term>Hérissons</term><term>Infestations par les puces</term><term>Maladies des chiens</term><term>Siphonaptera</term><term>Vecteurs insectes</term><term>Éthanol</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Algérie</term><term>Espagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Flea identification is a significant issue because some species are considered as important vectors of several human pathogens that have emerged or re-emerged recently, such as Bartonella henselae (Rhizobiales: Bartonellaceae) and Rickettsia felis (Rickettsiales: Rickettsiaceae). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been evaluated in recent years for the identification of multicellular organisms, including arthropods. A preliminary study corroborated the usefulness of this technique for the rapid identification of fleas, creating a preliminary database containing the spectra of five species of flea. However, longterm flea preservation in ethanol did not appear to be an adequate method of storage in the context of specimen identification by MALDI-TOF MS profiling. The goal of the present work was to assess the performance of MALDI-TOF MS in the identification of seven flea species [Ctenocephalides felis (Siphonaptera: Pulicidae), Ctenocephalides canis, Pulex irritans (Siphonaptera: Pulicidae), Archaeopsylla erinacei (Siphonaptera: Pulicidae), Leptopsylla taschenbergi (Siphonaptera: Ceratophyllidae), Stenoponia tripectinata (Siphonaptera: Stenoponiidae) and Nosopsyllus fasciatus (Siphonaptera: Ceratophyllidae)] collected in the field and stored in ethanol for different periods of time. The results confirmed that MALDI-TOF MS can be used for the identification of wild fleas stored in ethanol. Furthermore, this technique was able to discriminate not only different flea genera, but also the two congeneric species C. felis and C. canis.</div></front></TEI><affiliations><list><country><li>Algérie</li><li>Espagne</li><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement></list><tree><country name="Espagne"><noRegion><name sortKey="Zurita, A" sort="Zurita, A" uniqKey="Zurita A" first="A" last="Zurita">A. Zurita</name></noRegion><name sortKey="Callej N, R" sort="Callej N, R" uniqKey="Callej N R" first="R" last="Callej N">R. Callej N</name><name sortKey="Cutillas, C" sort="Cutillas, C" uniqKey="Cutillas C" first="C" last="Cutillas">C. Cutillas</name></country><country name="Algérie"><noRegion><name sortKey="Djeghar, R" sort="Djeghar, R" uniqKey="Djeghar R" first="R" last="Djeghar">R. Djeghar</name></noRegion></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Parola, P" sort="Parola, P" uniqKey="Parola P" first="P" last="Parola">P. Parola</name></region><name sortKey="Laroche, M" sort="Laroche, M" uniqKey="Laroche M" first="M" last="Laroche">M. Laroche</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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